Ligases & NA Modifying Enzymes

biotechrabbit™ T4 DNA Ligase Rapid is an exceptionally pure, highly concentrated ligase for applications in which high enzyme concentrations are required. It is especially recommended for fast ligations.

T4 DNA Ligase Rapid is supplied with 5× Rapid Ligation Buffer containing PEG for fast 5–10 minutes ligation, ligation of low-concentration or blunt-end DNA.

T4 DNA Ligase catalyzes the formation of a phosphodiester bond between the terminal 5′ phosphate and the 3′ hydroxyl groups of duplex DNA or RNA. The enzyme efficiently joins blunt and cohesive ends and repairs single stranded nicks in duplex DNA, RNA or DNA–RNA hybrids.

T4 DNA Ligase Rapid, 600 U/µl
Features
-Exceptionally pure high-concentration T4 DNA Ligase
-Perfect for rapid ligation

Applications
-Blunt and sticky end DNA ligation for cloning
-Joining of linkers or adaptors to double-stranded DNA
-Self-circularization of linear DNA

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