cDNA and ORF clones provide a quick and accessible method for obtaining gene content for many in vitro and cell culture overexpression applications. The coding sequence is cloned into an expression vector under control of a strong promoter and then delivered to cells of interest by plasmid transfection or lentiviral transduction.

cDNA are ideal for overexpressing a gene in the context of native regulation, while ORFs have both 5′ and 3′ UTRs removed providing a shortcut to protein expression. They are most commonly used for gene overexpression studies, RNAi rescue experiments, structural analysis, visualization of protein localization, biochemical purification, and identification of interacting proteins through co-immunoprecipitation.

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